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KMID : 0380019950100010009
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 1 p.9 ~ p.14
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Continuous Production of Fructooligosaccharides from Sucrose by a Dual Immobilized Enzyme System of Fructosyltransferase and Glucose Isomerase
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Abstract
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Continuous production of fructooligosaccharides from sucrose by a dual immobilized enzyme system of fructosyltransferase and glucose isomerase was studied in a column reactor. The optimal tempera- . ture and pH of the immobilized fructosyltransferase were 65°C and 5.5, respectively. The activity of glucose isomerase was favorable as temperature and pH were increased within the ranges examined. Howeve;both the immobilized enzymes were thermally unstable over 501C, suggesting that long-term operation of the dual immobilized enzyme column should be carried out below 501C. The optimum packing ratio of fructosyltransferase to glucose isomerase was found to be around 5/3. Under the optimized reaction conditions, the dual enzyme column was successfully operated for 40 days without any loss of initial enzyme activities, yielding 66% of fructooligosaccharides. Furthermore, the relative sweetness of fructooligosaccharides produced by a dual emzyme system was enhanced by-6% compared with that of fructosyltransf erase alone.
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